I actually have all my cameras on PnP (out of laziness really) and haven't had the gateway overwritten by the NVR even after multiple reboots of the NVR/Camera/Both. Though I agree that manual would be the safest bet to ensure everything is working 100%.
That's interesting, I can open up the camera stream the exact same way that alastairstevenson did using rtsp://192.168.254.X:554. So just to clarify, you're saying you cannot access rtsp://192.168.254.X:554 (the stream) but you can access the camera web interface using http://192.168.254.X:80?
Have you checked that your RTSP on the camera is actually 554? I've seen some cases where it was set to 10544 for some reason. Otherwise, maybe it could be your router? You could rule that out by connecting your computer directly to one of the camera ports, changing your computer ip to 192.168.254.128 and trying to open the stream at rtsp://192.168.254.X:554. Those two points considered, it may actually be that Hikvision changed their implementation of Virtual-Host between firmwares and that your version actually doesn't bridge the 554 port.
You understood me correct. I can access the camera web interface using http://192.168.254.2 but I can not access the videostream using rtsp://192.168.254.2:65001 or rtsp://192.168.254.2:65001/Streaming/Channels/101 from the browser or VLC.
Also my camera rtsp is 65001 and not 554. I believe it should be 65001 in order for the virtual host to operate. Do you imply that rtsp works only through port 554 and not 65001?
I will try later your suggestion to connect the computer directly to the camera ports of the NVR.